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Jing Yong Ye
photo of Jing Yong  Ye

Associate Research Scientist

office : 230 E.R.B. II
address : 2200 Bonisteel Blvd. - Ann Arbor, MI 48109-2099
email : jyye@eecs.umich.edu
phone : (734)936-0248 or (734)936-0248
fax : (734)763-4876

Research Group : Ultrafast Science

Publications : Reference List (103 listed)

Research Interests

The general focus of my research is the development and applications of ultrasensitive and ultrafast laser-based detection strategies to the problems in condensed matter physics, biophysics and analytical chemistry.

My present research involves in use of novel materials for second- and third-harmonic imaging of biological materials, novel techniques for biological fluorescence lifetime imaging, and adaptive optics for improved confocal imaging.

I also work on single molecule fluorescence imaging and spectroscopy. Unlike the conventional experiment in condensed matter that measures the average behavior of a huge number of molecules, single molecule measurement reveals detailed properties of individual molecules free from ensemble averaging. Many interesting phenomena unprecedentedly become observable. Using this technique one can gain an in-depth view of the nature of physical processes, chemical reactions and biological systems. I investigated a dye molecule system with a flexible molecular structure at a single-molecule level. The site-dependent nonradiative process of the individual dye molecules sensitively revealed the heterogeneous interactions between the dye molecule and its local environment. For a biologically important system, the interaction of a fluorescent nucleotide analogue with the Klenow fragment of DNA polymerase I was visualized at a single-molecule level. Furthermore, light-induced formation of individual nucleotide-enzyme complexes was observed in real time and in situ for the first time.

Femtosecond laser system provides us a powerful tool to investigate ultrafast dynamics through direct observation. Combining the ultrafast and ultrasensitive techniques, I succeeded in time-resolved fluorescence measurements at a single-molecule level.

Enhancement of weak fluorescence is another interesting subject, especially, for two-photon excited fluorescence, because two-photon absorption cross section of most organic molecules is extremely small. Using a one-dimensional photonic crystal, I observed a large enhancement of two-photon excited fluorescence of 2-aminopurine doped in a thin film as a defect layer in the photonic crystal. I further applied a novel idea to enhance fluorescence imaging by a photonic crystal and is applying for a patent for the method.

Besides light microscopy, atomic force microscopy (AFM) has a dramatic impact on many fields owing to its high spatial resolution. DNA is one of the most extensively studied molecules to understand its structure and interactions with protein molecules. However, long DNA molecules are subject to entanglements and aggregations, which make measurements difficult or even impossible, because AFM is a surface-based technique. The sample preparation, therefore, is extremely important. I developed an effective approach to stretching DNA molecules by spin-coating technique for sensitive AFM measurement.

Moreover, I have also conducted researches on microscopic dynamics of phase transition, ultrafast dynamics of TPM dyes, spectroscopic properties and biological activities of nucleotide, interaction of fluorescent analog with enzymes, and lasing without population inversion, etc.


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