About the Event
Photodynamic therapy (PDT) is a technology getting matured in 1980s for cancer treatment and receiving increasing attention nowadays for its intrinsic advantages over other kinds of cancer control strategies. In this work we developed a microfluidic platform for high-throughput PDT efficacy screening, which integrates various therapeutic factors and provides a comprehensive profile of photosensitizer efficacy change under different PDT conditions. Based on a triple microfluidic layer scheme, microenvironment controls of therapeutic factors including drug concentration, incubation time, oxygen level, flow shear stress and illumination dose (fluence) are included during the PDT efficacy test. And based on integrated gradient generation and combination of different therapeutic factors, high-throughput PDT efficacy test of more than 1,000 conditions can be carried out within an active area of 5╳5 mm2 of one chip in one run. This platform also providing compatibility with different cell types, photosensitizer generations and in vitro drug screening models. Computer program aid data collection and analysis enables a straightforward demonstration of PDT efficacy changes under different treatment conditions. This ultra-rapid, high throughput capable microfluidic platform will enable speedy developments of novel photosensitizers, expansion PDT application in cancer treatments and optimization of clinical PDT protocols.